01700nas a2200217   4500008004100000245008200041210006900123260000800192300001400200490000600214520101700220653001901237100001301256700001701269700001401286700001501300700001701315700001601332700001801348856011601366       2010                        eng d00aIn vitro liver model using microfabricated scaffolds in a modular bioreactor.0 aIn vitro liver model using microfabricated scaffolds in a modula  cFeb  a232–2410 v53 a<p>Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cell interactions are mediated by the architecture of the substrate, while the endogenous biochemical functions are regulated by a sustainable supply of nutrients and interstitial-like flow. Thus, a combination of 3-D scaffolds and dynamic flow conditions are both important for the development of a hepatic tissue model for applications in drug testing and regenerative medicine.</p>
10aBioengineering1 aVinci, B1 aCavallone, D1 aVozzi, G.1 aMazzei, D.1 aDomenici, C.1 aBrunetto, M1 aAhluwalia, A.  uhttp://175973.jxltd.asia/publications/vitro-liver-model-using-microfabricated-scaffolds-modular-bioreactor.html