01836nas a2200205 4500008004100000245007900041210006900120260001200189520120800201653001901409100001801428700001401446700001401460700001701474700001501491700001901506700001501525700001301540856007701553 2018 eng d00aSystemic and vascular inflammation in an in-vitro model of central obesity0 aSystemic and vascular inflammation in an invitro model of centra c02/20183 a

Metabolic disorders due to over-nutrition are a major global health problem, often associated with obesity and related morbidities. Obesity is peculiar to humans, as it is associated with lifestyle and diet, and so difficult to reproduce in animal models. Here we describe a model of human central adiposity based on a 3-tissue system consisting of a series of interconnected fluidic modules. Given the causal link between obesity and systemic inflammation, we focused primarily on pro-inflammatory markers, examining the similarities and differences between the 3-tissue model and evidence from human studies in the literature. When challenged with high levels of adiposity, the in-vitro system manifests cardiovascular stress through expression of E-selectin and von Willebrand factor as well as systemic inflammation (expressing IL-6 and MCP-1) as observed in humans. Interestingly, most of the responses are dependent on the synergic interaction between adiposity and the presence of multiple tissue types. The set-up has the potential to reduce animal experiments in obesity research and may help unravel specific cellular mechanisms which underlie tissue response to nutritional overload.

10aBioengineering1 aAhluwalia, A.1 aMisto, A.1 aVozzi, G.1 aMagliaro, C.1 aMattei, G.1 aMC, Marescotti1 aAVOGARO, A1 aIori, E. uhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.019282402116nas a2200193 4500008004100000245009500041210006900136260001200205520148600217653001901703100001501722700001701737700001401754700002501768700001401793700002001807700001801827856007701845 2017 eng d00aOn the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids0 aadhesioncohesion balance and oxygen consumption characteristics  c03/20173 a

Liver organoids (LOs) are of interest in tissue replacement, hepatotoxicity and pathophysiological studies. However, it is still unclear what triggers LO self-assembly and what the optimal environment is for their culture. Hypothesizing that LO formation occurs as a result of a fine balance between cell-substrate adhesion and cell-cell cohesion, we used 3 cell types (hepatocytes, liver sinusoidal endothelial cells and mesenchymal stem cells) to investigate LO self-assembly on different substrates keeping the culture parameters (e.g. culture media, cell types/number) and substrate stiffness constant. As cellular spheroids may suffer from oxygen depletion in the core, we also sought to identify the optimal culture conditions for LOs in order to guarantee an adequate supply of oxygen during proliferation and differentiation. The oxygen consumption characteristics of LOs were measured using an O2 sensor and used to model the O2 concentration gradient in the organoids. We show that no LO formation occurs on highly adhesive hepatic extra-cellular matrix-based substrates, suggesting that cellular aggregation requires an optimal trade-off between the adhesiveness of a substrate and the cohesive forces between cells and that this balance is modulated by substrate mechanics. Thus, in addition to substrate stiffness, physicochemical properties, which are also critical for cell adhesion, play a role in LO self-assembly.

10aBioengineering1 aMattei, G.1 aMagliaro, C.1 aGiusti, S1 aRamachandran, S., D.1 aHeinz, S.1 aBraspenning, J.1 aAhluwalia, A. uhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.017320602255nas a2200157 4500008004100000245012100041210006900162260001200231520172100243653001901964100001501983700001701998700001502015700001802030856004902048 2017 eng d00aDecellularized Human Liver Is Too Heterogeneous for Designing a Generic Extracellular Matrix Mimic Hepatic Scaffold.0 aDecellularized Human Liver Is Too Heterogeneous for Designing a  c05/20173 a

Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved. However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases. It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds. The intention of this work was to obtain quantitative design specifications for fabricating scaffolds for hepatic tissue engineering using human livers as a template. To this end, hepatic samples from five patients scheduled for hepatic resection were decellularized using a protocol shown to reproducibly conserve matrix composition and microstructure in porcine livers. The decellularization outcome was evaluated through histological and quantitative image analyses to evaluate cell removal, protein, and glycosaminoglycan content per unit area. Applying the same decellularization protocol to human liver samples obtained from five different patients yielded five different outcomes. Only one liver out of five was completely decellularized, while the other four showed different levels of remaining cells and matrix. Moreover, protein and glycosaminoglycan content per unit area after decellularization were also found to be patient- (or donor-) dependent. This donor-to-donor variability of human livers thus precludes their use as templates for engineering a generic "one-size fits all" ECM-mimic hepatic scaffold.

10aBioengineering1 aMattei, G.1 aMagliaro, C.1 aPirone, A.1 aAhluwalia, A. uhttps://www.ncbi.nlm.nih.gov/pubmed/2854340302803nas a2200181 4500008004100000245012600041210006900167520217400236653001902410100001702429700002002446700001502466700001902481700001502500700001502515700001802530856007302548 2016 eng d00aClarifying CLARITY: Quantitative Optimization of the Diffusion Based Delipidation Protocol for Genetically Labeled Tissue0 aClarifying CLARITY Quantitative Optimization of the Diffusion Ba3 a

Tissue clarification has been recently proposed to allow deep tissue imaging without light scattering. The clarification parameters are somewhat arbitrary and dependent on tissue type, source and dimension: every laboratory has its own protocol, but a quantitative approach to determine the optimum clearing time is still lacking. Since the use of transgenic mouse lines that express fluorescent proteins to visualize specific cell populations is widespread, a quantitative approach to determine the optimum clearing time for genetically labeled neurons from thick murine brain slices using CLARITY2 is described. In particular, as the main objective of the delipidation treatment is to clarify tissues, while limiting loss of fluorescent signal, the “goodness” of clarification was evaluated by considering the bulk tissue clarification index (BTCi) and the fraction of the fluorescent marker retained in the slice as easily quantifiable macroscale parameters. Here we describe the approach, illustrating an example of how it can be used to determine the optimum clearing time for 1 mm-thick cerebellar slice from transgenic L7GFP mice, in which Purkinje neurons express the GFP (green fluorescent protein) tag. To validate the method, we evaluated confocal stacks of our samples using standard image processing indices (i.e., the mean pixel intensity of neurons and the contrast-to-noise ratio) as figures of merit for image quality. The results show that detergent-based delipidation for more than 5 days does not increase tissue clarity but the fraction of GFP in the tissue continues to diminish. The optimum clearing time for 1 mm-thick slices was thus identified as 5 days, which is the best compromise between the increase in light penetration depth due to removal of lipids and a decrease in fluorescent signal as a consequence of protein loss: further clearing does not improve tissue transparency, but only leads to more protein removal or degradation. The rigorous quantitative approach described can be generalized to any clarification method to identify the moment when the clearing process should be terminated to avoid useless protein loss.

10aBioengineering1 aMagliaro, C.1 aCallara, A., L.1 aMattei, G.1 aMorcinelli, M.1 aViaggi, C.1 aVaglini, F1 aAhluwalia, A. uhttp://journal.frontiersin.org/article/10.3389/fnins.2016.00179/full01784nas a2200121 4500008004100000245009100041210006900132520133800201653001901539100001801558700001501576856007101591 2016 eng d00aSample, testing and analysis variables affecting liver mechanical properties: A review0 aSample testing and analysis variables affecting liver mechanical3 a

Given the critical role of liver mechanics in regulating cell response and directing the development of tissue fibrosis, accurately characterising its mechanical behaviour is of relevance for both diagnostic purposes as well as for tissue engineering and for the development of in-vitro models. Determining and quantifying the mechanical behaviour of soft biological tissues is, however, highly challenging due to their intrinsic labile nature. Indeed, a unique set of values of liver mechanical properties is still lacking to date; testing conditions can significantly affect sample status and hence the measured behaviour and reported results are strongly dependent on the adopted testing method and configuration as well as sample type and status. This review aims at summarising the bulk mechanical properties of liver described in the literature, discussing the possible sources of variation and their implications on the reported results. We distinguish between the intrinsic mechanical behaviour of hepatic tissue, which depends on sample variables, and the measured mechanical properties which also depend on the testing and analysis methods. Finally, the review provides guidelines on tissue preparation and testing conditions for generating reproducible data which can be meaningfully compared across laboratories.

10aBioengineering1 aAhluwalia, A.1 aMattei, G. uhttp://www.sciencedirect.com/science/article/pii/S174270611630460301564nas a2200133 4500008004100000022001300041245009100054210006900145520109700214653001901311100001501330700001401345856007101359 2015 eng d a0098135400aCFD modelling of a mixing chamber for the realisation of functionally graded scaffolds0 aCFD modelling of a mixing chamber for the realisation of functio3 a

Biological tissues are characterised by spatially distributed gradients, intricately linked with functions. It widely accepted that ideal tissue engineered scaffolds should exhibit similar functional gradients to promote successful tissue regeneration. Focusing on bone, in previous work we proposed simple methods to obtain osteochondral functionally graded scaffolds (FGSs), starting from homogeneous suspensions of hydroxyapatite (HA) particles in gelatin solutions. With the main aim of developing an automated device to fabricate FGSs, this work is focused on designing a stirred tank to obtain homogeneous HA-gelatin suspensions. The HA particles transport within the gelatin solution was investigated through computational fluid dynamics (CFD) modelling. First, the steady-state flow field was solved for the continuous phase only. Then, it was used as a starting point for solving the multi-phase transient simulation. CFD results showed that the proposed tank geometry and setup allow for obtaining a homogeneous suspension of HA micro-particles within the gelatin solution.

10aBioengineering1 aMattei, G.1 aVozzi, G. uhttp://www.sciencedirect.com/science/article/pii/S009813541500282300612nas a2200181 4500008004100000022001400041245012700055210006900182300001000251490000600261653001900267100001500286700001700301700001500318700001800333700002500351856005400376 2015 eng d a2045-232200aDecoupling the role of stiffness from other hydroxyapatite signalling cues in periosteal derived stem cell differentiation0 aDecoupling the role of stiffness from other hydroxyapatite signa a107780 v510aBioengineering1 aMattei, G.1 aFerretti, C.1 aTirella, A1 aAhluwalia, A.1 aMattioli-Belmonte, M uhttp://www.nature.com/doifinder/10.1038/srep1077800588nas a2200157 4500008004100000245009000041210006900131260001200200653001900212100001700231700001500248700001500263700001500278700001800293856011900311 2015 eng d00a HisTOOLogy: an open-source tool for quantitative analysis of histological sections 0 aHisTOOLogy an opensource tool for quantitative analysis of histo c08/201510aBioengineering1 aMagliaro, C.1 aTirella, A1 aMattei, G.1 aPirone, A.1 aAhluwalia, A. uhttp://175973.jxltd.asia/publications/histoology-open-source-tool-quantitative-analysis-histological-sections.html00592nas a2200169 4500008004100000022001300041245012900054210006900183300001400252490000700266653001900273100001500292700001400307700001700321700001800338856006600356 2015 eng d a1751616100aThe nano-epsilon dot method for strain rate viscoelastic characterisation of soft biomaterials by spherical nano-indentation0 ananoepsilon dot method for strain rate viscoelastic characterisa a150–1590 v5010aBioengineering1 aMattei, G.1 aGruca, G.1 aRijnveld, N.1 aAhluwalia, A. uhttp://linkinghub.elsevier.com/retrieve/pii/S175161611500208800590nas a2200193 4500008004100000022001300041245005300054210005000107300001600157490000700173653001900180100001500199700001600214700001600230700001500246700001500261700001800276856010200294 2015 eng d a1550703300aNano-in-Micro Self-Reporting Hydrogel Constructs0 aNanoinMicro SelfReporting Hydrogel Constructs a1451–14600 v1110aBioengineering1 aTirella, A1 aLa Marca, M1 aBrace, L.A.1 aMattei, G.1 aAylott, J.1 aAhluwalia, A. uhttp://openurl.ingenta.com/content/xref?genre=article&issn=1550-7033&volume=11&issue=8&spage=145100511nas a2200157 4500008004100000245009700041210006900138260001200207490001100219653001900230100001500249700001800264700001700282700001800299856003600317 2015 eng d00aProfile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome0 aProfile analysis of hepatic porcine and murine brain tissue slic c04/20150 v3:e93210aBioengineering1 aMattei, G.1 aCristiani, I.1 aMagliaro, C.1 aAhluwalia, A. uhttps://peerj.com/articles/932/02055nas a2200169 4500008004100000022001400041245009100055210006900146300001400215490000600229520154100235653001901776100001501795700001401810700001801824856004301842 2014 eng d a2227-971700aDesign Criteria for Generating Physiologically Relevant In Vitro Models in Bioreactors0 aDesign Criteria for Generating Physiologically Relevant In Vitro a548–5690 v23 a

In this paper, we discuss the basic design requirements for the development of physiologically meaningful in vitro systems comprising cells, scaffolds and bioreactors, through a bottom up approach. Very simple micro- and milli-fluidic geometries are first used to illustrate the concepts, followed by a real device case-study. At each step, the fluidic and mass transport parameters in biological tissue design are considered, starting from basic questions such as the minimum number of cells and cell density required to represent a physiological system and the conditions necessary to ensure an adequate nutrient supply to tissues. At the next level, we consider the use of three-dimensional scaffolds, which are employed both for regenerative medicine applications and for the study of cells in environments which better recapitulate the physiological milieu. Here, the driving need is the rate of oxygen supply which must be maintained at an appropriate level to ensure cell viability throughout the thickness of a scaffold. Scaffold and bioreactor design are both critical in defining the oxygen profile in a cell construct and are considered together. We also discuss the oxygen-shear stress trade-off by considering the levels of mechanical stress required for hepatocytes, which are the limiting cell type in a multi-organ model. Similar considerations are also made for glucose consumption in cell constructs. Finally, the allometric approach for generating multi-tissue systemic models using bioreactors is described.

10aBioengineering1 aMattei, G.1 aGiusti, S1 aAhluwalia, A. uhttp://www.mdpi.com/2227-9717/2/3/548/02063nas a2200193 4500008003900000245010100039210006900140260002700209300001100236520139000247653001901637100001401656700001501670700001401685700001801699700001501717700001401732856012301746 2014 d00aThe need of innovative technologies for new 3D relevant in-vitro models and the answer of Ivtech0 aneed of innovative technologies for new 3D relevant invitro mode aPrague, Czech Republic a45–63 a

New relevant in-vitro models are priorities in pharmaco-toxicology, cosmetic and food research to reduce the animal tests. Therefore, invivo models show ethical issues, are not time and cost effective and are progressively showing scientific limitations: for instance they fail in detection of pathogens that are species specific (Mazzoleni et al., 2009). The search of more relevant pre-clinical models forced the researcher to move from 2D to 3D in-vitro models in order to maintain the phenotype of cells (Lovit et al., 2013; Mattei et al., 2014). Even if the significant progress in material science, the metabolic requirement of 3D tissues is higher than a 2D culture and the scaffold is a limitation in nutrients transport. Dynamic cell culture chambers are then required to assure the gas/nutrient supply, waste elimination, mechanical stimulation of cells, study of cross talk between different tissues and real time monitoring of cells. Nowadays the only systems that meet all these specifications are the Ivtech technologies. Ivtech is an innovative Italian start-up that grows up to solve the needs of in-vitro experts, offering and customizing several type of transparent, dynamic and modular cell culture systems, organizing workshops and training. The goal is to expand the 3D approach and permits a significant evolution towards highly relevant in-vitro models.

10aBioengineering1 aSbrana, T1 aMattei, G.1 aGiusti, S1 aAhluwalia, A.1 aDagmar, J.1 aHorst, S. uhttp://175973.jxltd.asia/publications/need-innovative-technologies-new-3d-relevant-vitro-models-and-answer-ivtech.html00656nas a2200193 4500008004100000022001400041245008000055210006900135300001200204490000600216653001900222100001300241700001500254700001900269700001700288700002500305700001400330856011800344 2013 eng d a0928-493100aBone scaffolds with homogeneous and discrete gradient mechanical properties0 aBone scaffolds with homogeneous and discrete gradient mechanical a28–360 v110aBioengineering1 aJelen, C1 aMattei, G.1 aMontemurro, F.1 aDe Maria, C.1 aMattioli-Belmonte, M1 aVozzi, G. uhttp://175973.jxltd.asia/publications/bone-scaffolds-homogeneous-and-discrete-gradient-mechanical-properties.html00662nas a2200157 4500008003900000245014100039210006900180260001800249653001900267100001500286700001500301700002500316700001600341700001800357856012900375 2013 d00aGelatin-Hydroxyapatite hydrogels to investigate periosteal derived progenitor cells (PDPCs) response for tissue engineering applications0 aGelatinHydroxyapatite hydrogels to investigate periosteal derive aLiverpool, UK10aBioengineering1 aMattei, G.1 aTirella, A1 aMattioli-Belmonte, M1 aFerretti, C1 aAhluwalia, A. uhttp://175973.jxltd.asia/publications/gelatin-hydroxyapatite-hydrogels-investigate-periosteal-derived-progenitor-cells-pdpcs00592nas a2200157 4500008003900000245009400039210006900133260001100202300001100213653001900224100001700243700001500260700001500275700001800290856012600308 2013 d00a{SpHyGa: fabrication of hepatic lobule replicas using tissue derived material composites}0 aSpHyGa fabrication of hepatic lobule replicas using tissue deriv aMadrid aID 52010aBioengineering1 aMarca, La, M1 aTirella, A1 aMattei, G.1 aAhluwalia, A. uhttp://175973.jxltd.asia/publications/sphyga-fabrication-hepatic-lobule-replicas-using-tissue-derived-material-composites01842nas a2200145 4500008004100000022001400041245008200055210006900137520137500206653001901581100001501600700001501615700001801630856004801648 2013 eng d a1552-496500a{Strain rate viscoelastic analysis of soft and highly hydrated biomaterials.}0 aStrain rate viscoelastic analysis of soft and highly hydrated bi3 a

Measuring the viscoelastic behaviour of highly hydrated biological materials is challenging because of their intrinsic softness and labile nature. In these materials it is difficult to avoid pre-stress and therefore to establish precise initial stress and strain conditions for lumped parameter estimation using creep or stress-relaxation tests. We describe a method ($ε$M or epsilon dot method) for deriving the viscoelastic parameters of soft hydrated biomaterials which avoids pre-stress and can be used to rapidly test degradable samples. Standard mechanical tests are first performed compressing samples using different strain rates. The dataset obtained is then analysed to mathematically derive the material's viscoelastic parameters. In this work a stable elastomer, PDMS, and a labile hydrogel, gelatin, were first tested using the $ε$M, in parallel stress-relaxation was used to compare lumped parameter estimation. After demonstrating that the elastic parameters are equivalent and that the estimation of short time constants is more precise using the proposed method, the viscoelastic behaviour of porcine liver was investigated using this approach. The results show that the constitutive parameters of hepatic tissue can be quickly quantified without the application of any pre-stress and before the onset of time dependent degradation phenomena.

10aBioengineering1 aTirella, A1 aMattei, G.1 aAhluwalia, A. uhttp://www.ncbi.nlm.nih.gov/pubmed/2394605400560nas a2200145 4500008004100000245008700041210006900128260001600197300001400213653001900227100001400246700001400260700001500274856012500289 2012 eng d00aCharacterization and modeling of mechanical behavior a discrete gradient structure0 aCharacterization and modeling of mechanical behavior a discrete  aRoma, Italy a130 - 13110aBioengineering1 aCaputo, M1 aVozzi, G.1 aMattei, G. uhttp://175973.jxltd.asia/publications/characterization-and-modeling-mechanical-behavior-discrete-gradient-structure.html02061nas a2200169 4500008003900000245006000039210005800099260002000157520150900177653001901686100001501705700001801720700001601738700002501754700001501779856009701794 2012 d00aContinuous functionally graded materials (cFGMs) for TE0 aContinuous functionally graded materials cFGMs for TE aVienna, Austria3 a

Biological structures are not uniform but possess spatially distributed functions and properties, or functional gradients. To ensure functional, mechanical and structural integration, a tissue engineered (TE) scaffold has to reproduce these functional gradients. However the fabrication of functionally graded materials is challenging and usually an experimental trial-and-error approach is used. In this work we present a controlled method for the fabrication of cFGMs using the gravitational sedimentation of discrete solid particles within a primary fluid phase. To have an overall control over particle distribution, a time-varying dynamic viscosity solution (i.e. thermo-sensitive) was used as fluid phase. Computational fluid dynamic models were developed to have a fine control over particle distribution. Biomimetic osteochondral cFGMs scaffolds were fabricated using hydroxyapatite (HA) and gelatin. Glutaraldehyde was used to covalently bind gelatin-HA graded scaffolds. Mechanical properties were measured and correlated as a function of HA volume fraction. SEM-EDX analysis was used to further characterise HA content and its distribution within gelatin-HA cFGMs. Finally gelatin-HA cFGMs scaffold were seeded using periosteum derived progenitor cells, to investigate how the HA gradient modulates cell response. This approach represents an innovative yet simple tool for the fabrication of tailored cFGMs with biologically and physiologically relevant gradients for TE applications.

10aBioengineering1 aMattei, G.1 aAhluwalia, A.1 aFerretti, C1 aMattioli-Belmonte, M1 aTirella, A uhttp://175973.jxltd.asia/publications/continuous-functionally-graded-materials-cfgms-te.html00578nas a2200133 4500008004100000245013200041210006900173300001300242653001900255100001500274700001500289700001800304856012200322 2012 eng d00aFunctionally Graded Materials (FGMs) with predictable and controlled gradient profiles: computational modelling and realisation0 aFunctionally Graded Materials FGMs with predictable and controll aaccepted10aBioengineering1 aMattei, G.1 aTirella, A1 aAhluwalia, A. uhttp://175973.jxltd.asia/publications/functionally-graded-materials-fgms-predictable-and-controlled-gradient-profiles01839nas a2200145 4500008003900000245008600039210006900125260001600194520129800210653001901508100001501527700001501542700001801557856011801575 2012 d00aNew method for the viscoelastic analysis of soft and highly hydrated biomaterials0 aNew method for the viscoelastic analysis of soft and highly hydr aRome, Italy3 a

The role of materials mechanical properties is a new frontier for the evaluation of cell/material interaction, as well as for the determination of healthy/pathological state of a tissue. In these sense there is the need to have a unique method to measure materials viscoelastic properties. However, concerning with soft and highly hydrated constructs, the experimental set-up to precisely measure these properties is challenging because of the difficulty in defining zero stress or strain. To overcome these problems, we propose a novel and unique testing and data analysis technique (ERM) to derive materials viscoelastic properties. Results derived with this method can be compared to the ones obtained with standard testing techniques for viscoelastic materials. Pre-conditioning problems of testing soft and floppy materials are thus overcome, giving rise to have an accurate measure of viscous and elastic moduli of both hydrated materials and soft biological tissues. Small variations of measured properties can be also monitored with high precision, allowing a deeper investigation on the role of the scaffolding material or of tissue’s extracellular matrix (respectively in cell culture systems or in biomechanics measurements for the characterisation of soft tissues).

10aBioengineering1 aTirella, A1 aMattei, G.1 aAhluwalia, A. uhttp://175973.jxltd.asia/publications/new-method-viscoelastic-analysis-soft-and-highly-hydrated-biomaterials.html00670nas a2200169 4500008003900000245006500039210006500104260002400169520010200193653001900295100001500314700001500329700001900344700001400363700001800377856010500395 2011 d00aModelling and realisation of smart graded hydrogel scaffolds0 aModelling and realisation of smart graded hydrogel scaffolds aMontpellier, France3 a

European Congress and Exhibition on Advanced Materials and Processes - Poster F12-P-2-08

10aBioengineering1 aTirella, A1 aMattei, G.1 aMontemurro, F.1 aVozzi, G.1 aAhluwalia, A. uhttp://175973.jxltd.asia/publications/modelling-and-realisation-smart-graded-hydrogel-scaffolds.html00570nas a2200145 4500008004100000022001400041245008600055210006900141653001900210100001500229700001900244700002500263700001400288856012200302 2010 eng d a2036-679500aNovel injectable hydrogel scaffold for cartilage repair based on natural polymers0 aNovel injectable hydrogel scaffold for cartilage repair based on10aBioengineering1 aMattei, G.1 aMontemurro, F.1 aMattioli-Belmonte, M1 aVozzi, G. uhttp://175973.jxltd.asia/publications/novel-injectable-hydrogel-scaffold-cartilage-repair-based-natural-polymers.html00591nas a2200145 4500008004100000245011400041210006900155300000800224653001900232100001500251700001900266700001800285700001400303856012800317 2008 eng d00aSviluppo e caratterizzazione meccanica di scaffold a base di collagene per la rigenerazione della cartilagine0 aSviluppo e caratterizzazione meccanica di scaffold a base di col a–10aBioengineering1 aMattei, G.1 aMontemurro, F.1 aAhluwalia, A.1 aVozzi, G. uhttp://175973.jxltd.asia/publications/sviluppo-e-caratterizzazione-meccanica-di-scaffold-base-di-collagene-la-rigenerazione